Overcoming multidrug resistance in human tumor cells using free and liposomally encapsulated antisense oligodeoxynucleotides.
Identifieur interne : 004514 ( Main/Exploration ); précédent : 004513; suivant : 004515Overcoming multidrug resistance in human tumor cells using free and liposomally encapsulated antisense oligodeoxynucleotides.
Auteurs : A R Thierry ; A. Rahman ; A. DritschiloSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 1993.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Cellules cancéreuses en culture, Données de séquences moléculaires, Expression des gènes, Glycoprotéines membranaires (génétique), Humains, Liposomes, Oligonucléotides antisens (), Oligonucléotides antisens (administration et posologie), Résistance aux substances, Séquence nucléotidique, Techniques in vitro.
- MESH :
- administration et posologie : Oligonucléotides antisens.
- génétique : ARN messager, Glycoprotéines membranaires.
- Cellules cancéreuses en culture, Données de séquences moléculaires, Expression des gènes, Humains, Liposomes, Oligonucléotides antisens, Résistance aux substances, Séquence nucléotidique, Techniques in vitro.
English descriptors
- KwdEn :
- ATP Binding Cassette Transporter, Subfamily B, Member 1, Base Sequence, Drug Resistance, Gene Expression, Humans, In Vitro Techniques, Liposomes, Membrane Glycoproteins (genetics), Molecular Sequence Data, Oligonucleotides, Antisense (administration & dosage), Oligonucleotides, Antisense (chemistry), RNA, Messenger (genetics), Tumor Cells, Cultured.
- MESH :
- chemical , administration & dosage : Oligonucleotides, Antisense.
- chemical , chemistry : Oligonucleotides, Antisense.
- chemical , genetics : Membrane Glycoproteins, RNA, Messenger.
- chemical : ATP Binding Cassette Transporter, Subfamily B, Member 1, Liposomes.
- Base Sequence, Drug Resistance, Gene Expression, Humans, In Vitro Techniques, Molecular Sequence Data, Tumor Cells, Cultured.
Abstract
Antisense oligonucleotides offer a molecular targeting tool for overcoming cellular multidrug resistance. In order to improve the in vitro and the in vivo transport of oligodeoxynucleotides, we developed a new liposomal delivery system, using the minimal volume entrapment (MVE) technique. We have demonstrated that cellular uptake and intracellular release of oligodeoxynucleotides were facilitated by delivery in liposomes. 15 mers cap phosphorothioate oligodeoxynucleotides complementary to the 5' end of the coding region or to a loop-forming site in the mdr-1 mRNA were encapsulated in liposomes by the MVE method. P-glycoprotein synthesis and doxorubicin resistance were greatly reduced by exposure of the multidrug resistant SKVLB cells to 5 microM liposomally encapsulated oligonucleotide. A lower effect was observed when free oligodeoxynucleotides were used. Oligomers antisense to the loop-forming site appeared to be more effective and more specific in modulating multidrug resistance than oligomers with antisense sequence to the 5' end coding region.
DOI: 10.1006/bbrc.1993.1142
PubMed: 8094959
Affiliations:
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Le document en format XML
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Dritschilo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8094959</idno><idno type="pmid">8094959</idno><idno type="doi">10.1006/bbrc.1993.1142</idno><idno type="wicri:Area/PubMed/Corpus">002925</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002925</idno><idno type="wicri:Area/PubMed/Curation">002925</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002925</idno><idno type="wicri:Area/PubMed/Checkpoint">002771</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002771</idno><idno type="wicri:Area/Ncbi/Merge">002964</idno><idno type="wicri:Area/Ncbi/Curation">002964</idno><idno type="wicri:Area/Ncbi/Checkpoint">002964</idno><idno type="wicri:doubleKey">0006-291X:1993:Thierry A:overcoming:multidrug:resistance</idno><idno type="wicri:Area/Main/Merge">004578</idno><idno type="wicri:Area/Main/Curation">004514</idno><idno type="wicri:Area/Main/Exploration">004514</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Overcoming multidrug resistance in human tumor cells using free and liposomally encapsulated antisense oligodeoxynucleotides.</title><author><name sortKey="Thierry, A R" sort="Thierry, A R" uniqKey="Thierry A" first="A R" last="Thierry">A R Thierry</name><affiliation><nlm:affiliation>Department of Radiation Medicine, Vincent T. Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, D.C. 20007.</nlm:affiliation><wicri:noCountry code="subField">D.C.</wicri:noCountry></affiliation></author><author><name sortKey="Rahman, A" sort="Rahman, A" uniqKey="Rahman A" first="A" last="Rahman">A. Rahman</name></author><author><name sortKey="Dritschilo, A" sort="Dritschilo, A" uniqKey="Dritschilo A" first="A" last="Dritschilo">A. Dritschilo</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ATP Binding Cassette Transporter, Subfamily B, Member 1</term><term>Base Sequence</term><term>Drug Resistance</term><term>Gene Expression</term><term>Humans</term><term>In Vitro Techniques</term><term>Liposomes</term><term>Membrane Glycoproteins (genetics)</term><term>Molecular Sequence Data</term><term>Oligonucleotides, Antisense (administration & dosage)</term><term>Oligonucleotides, Antisense (chemistry)</term><term>RNA, Messenger (genetics)</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Cellules cancéreuses en culture</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Glycoprotéines membranaires (génétique)</term><term>Humains</term><term>Liposomes</term><term>Oligonucléotides antisens ()</term><term>Oligonucléotides antisens (administration et posologie)</term><term>Résistance aux substances</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>ATP Binding Cassette Transporter, Subfamily B, Member 1</term><term>Liposomes</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Glycoprotéines membranaires</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Drug Resistance</term><term>Gene Expression</term><term>Humans</term><term>In Vitro Techniques</term><term>Molecular Sequence Data</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cancéreuses en culture</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Humains</term><term>Liposomes</term><term>Oligonucléotides antisens</term><term>Résistance aux substances</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antisense oligonucleotides offer a molecular targeting tool for overcoming cellular multidrug resistance. In order to improve the in vitro and the in vivo transport of oligodeoxynucleotides, we developed a new liposomal delivery system, using the minimal volume entrapment (MVE) technique. We have demonstrated that cellular uptake and intracellular release of oligodeoxynucleotides were facilitated by delivery in liposomes. 15 mers cap phosphorothioate oligodeoxynucleotides complementary to the 5' end of the coding region or to a loop-forming site in the mdr-1 mRNA were encapsulated in liposomes by the MVE method. P-glycoprotein synthesis and doxorubicin resistance were greatly reduced by exposure of the multidrug resistant SKVLB cells to 5 microM liposomally encapsulated oligonucleotide. A lower effect was observed when free oligodeoxynucleotides were used. Oligomers antisense to the loop-forming site appeared to be more effective and more specific in modulating multidrug resistance than oligomers with antisense sequence to the 5' end coding region.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Dritschilo, A" sort="Dritschilo, A" uniqKey="Dritschilo A" first="A" last="Dritschilo">A. Dritschilo</name><name sortKey="Rahman, A" sort="Rahman, A" uniqKey="Rahman A" first="A" last="Rahman">A. Rahman</name><name sortKey="Thierry, A R" sort="Thierry, A R" uniqKey="Thierry A" first="A R" last="Thierry">A R Thierry</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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